Narishige Pc10 Manual New | TOP – Pick |

The is a versatile, vertical-pull micropipette puller designed for the high-precision fabrication of glass microelectrodes and injection needles. This model replaces older semi-automatic units like the PP-830, offering a more automated and robust experience for researchers in biotechnology and electrophysiology. Core Operation Modes

Assuming you have just acquired a pristine, scanned, or reprinted manual, follow this checklist:

The Narishige PC-10 is a dual-stage glass micropipette puller widely utilized in electrophysiology, microinjection, and cellular manipulation. This comprehensive guide serves as an expanded manual and operational overview for the PC-10 system, detailing its technical specifications, installation protocols, step-by-step programming, and advanced troubleshooting techniques for new users and laboratory technicians. 1. Introduction and Technical Specifications

Before connecting power, check that the internal voltage setting matches your lab’s outlet voltage (100V-240V). The AC power inlet contains a voltage selector, which must be set correctly 1.2.5. Installing Glass Capillaries Place the glass capillary securely into the chucks.

: Clean blood or chemical stains with organic solvents like alcohol; do not boil for sterilization. Disassembly narishige pc10 manual new

Produces shorter, thicker, and stiffer tips.

The most critical section of the PC-10 manual is the calibration protocol. This includes:

: Pulls the glass capillary at one stretch, typically used for creating supple pipettes.

Clean a standard glass capillary tube with ethanol to remove dust and skin oils. This comprehensive guide serves as an expanded manual

When the first drop triggers the microswitch, the unit pauses briefly (allowing the glass to cool slightly) and then applies the second heating stage.

Set the first-stage heater value. Adjust the stop micrometer so the lower slider drops only 1 to 2 millimeters during the first burn. This thins the glass without breaking it.

For a standard (commonly used for patch pipettes with a 1–2 Set the toggle switch to 2-Stage .

: Compatible with borosilicate glass capillaries, such as the Narishige GD-1 with internal filaments. 3. Calibration & Setup (Manual Guidelines) The AC power inlet contains a voltage selector,

Expert users on platforms like ResearchGate suggest specific workflows:

This comprehensive manual provides the necessary steps to set up, calibrate, operate, and troubleshoot your Narishige PC-10 to ensure reproducible results in your laboratory. 1. Safety and Installation Guidelines

Pulled glass pipettes feature incredibly sharp tips that can easily puncture skin. Dispose of all glass shards and failed pipettes in a designated sharps container.

This guide serves as a detailed manual supplement for new users or those seeking a refresher on the operation, theory, and maintenance of the Narishige PC-10.